Quantitative analysis of fluorescent ligand binding to dopamine D

Autor:	Anni, Allikalt, Tõnis, Laasfeld, Mihkel, Ilisson, Sergei, Kopanchuk, Ago, Rinken
Rok vydání:	2020
Předmět:	Dopamine Carbocyanines Ligands Receptors Dopamine Machine Learning Kinetics HEK293 Cells Microscopy Fluorescence Spiperone Image Processing Computer-Assisted Dopamine Antagonists Humans Biological Assay Software Protein Binding
Zdroj:	The FEBS journalReferences. 288(5)
ISSN:	1742-4658
Popis:	Dopamine receptors are G protein-coupled receptors that have several essential functions in the central nervous system. A better understanding of the regulatory mechanisms of ligand binding to the receptor may open new possibilities to affect the downstream signal transduction pathways. The majority of the available ligand binding assays use either membrane preparations, cell suspensions, or genetically modified receptors, which may give at least partially incorrect understanding of ligand binding. In this study, we implemented an assay combining fluorescence and bright-field microscopy to measure ligand binding to dopamine D
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::2a84ff1cd3e878b0b362d0f828ddcc09 https://pubmed.ncbi.nlm.nih.gov/32783364 Zobrazit plný text záznamu Plný text