| Autor: |
V V, Ovod, L A, Mikhal'skiĭ, A E, Vershigora, R I, Gvozdiak |
| Rok vydání: |
1991 |
| Předmět: |
|
| Zdroj: |
Mikrobiologicheskii zhurnal. 53(1) |
| ISSN: |
0201-8462 |
| Popis: |
Water extract and salt-EDTA extract of Pseudomonas syringae, pv. atrofaciens cells were fractionated by ultracentrifugation with following salting out of ultracentrifugal supernatant by ammonium sulphate at 55% saturation (pH 4.5). The composition and distribution of LPS molecular forms were studied in the obtained fractions by means of electrophoresis in 10% polyacrylamide gel with 1% sodium dodecylsulphate when staining gels by silver nitrate and cumassi. It is shown that ultracentrifugal supernatant and a sediment as well as sulphate sediment contain S-LPS and R-LPS. SR-LPS is not differentiated. Sulphate supernatant does not contain the determinable amount of S-LPS but it is enriched by the proteins with molecular weights of 65-15 kDalton. S-LPS is localized in the gel area which corresponds to mobilities of polypeptides with molecular weights 130-45 KDalton and the number of monomeric links in O-specific chains of its molecules reaches 25-30. R-LPS migrates under electrophoresis in gel to the mobility zone of polypeptides with molecular weights 14.5-16 kDalton. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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