Detection of anaplastic lymphoma kinase (ALK) and nucleolar protein nucleophosmin (NPM)-ALK proteins in normal and neoplastic cells with the monoclonal antibody ALK1
| Autor: | K, Pulford, L, Lamant, S W, Morris, L H, Butler, K M, Wood, D, Stroud, G, Delsol, D Y, Mason |
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| Rok vydání: | 1997 |
| Předmět: |
Adult
Male Adolescent Lymphoma Oncogene Proteins Fusion Nerve Tissue Proteins Transfection Polymerase Chain Reaction Translocation Genetic Diagnosis Differential Immunoenzyme Techniques Mice Neoplasms Biomarkers Tumor Tumor Cells Cultured Animals Humans Anaplastic Lymphoma Kinase Child Aged Aged 80 and over Mice Inbred BALB C Antibodies Monoclonal Nuclear Proteins Receptor Protein-Tyrosine Kinases DNA Neoplasm Middle Aged Protein-Tyrosine Kinases Hodgkin Disease Neoplasm Proteins Organ Specificity Child Preschool Chromosomes Human Pair 2 Chromosomes Human Pair 5 Feasibility Studies Female Lymphoma Large B-Cell Diffuse Nucleophosmin |
| Zdroj: | Blood. 89(4) |
| ISSN: | 0006-4971 |
| Popis: | The t(2;5)(p23;q35) translocation, associated with anaplastic large-cell lymphoma (ALCL), results in the production of the nucleolar protein nucleophosmin-anaplastic lymphoma kinase (NPM-ALK) protein. This report describes an immunocytochemical study of the distribution of ALK and NPM-ALK proteins using a new monoclonal antibody, ALK1, that recognizes a formalin resistant epitope in both the 80-kD NPM-ALK chimeric and the 200-kD normal human ALK proteins. Cytoplasmic and nuclear labeling was seen in the t(2;5)+ SU-DHL-1 and Karpas 299 cell lines. Normal ALK protein expression was restricted to the central nervous system (in scattered neurons, glial cells, and endothelial cells). Two hundred and thirty-nine cases of lymphoma and 80 nonhematopoietic tumors were immunostained. Antibody ALK1 labeled 53.4% (39 of 73 cases) of CD30+ ALCL. A case of ALCL with a t(1;2) translocation was ALK1+. Three cases of CD30- ALCL with prominent nucleoli showed a unique pattern of coarse granular cytoplasmic labeling. All other tumors, including Hodgkin's disease and lymphomatoid papulosis, were ALK1-. These results indicate that reliable immunostaining of routine biopsy material for NPM-ALK and ALK proteins is feasible. Such analysis is of diagnostic importance, especially because t(2;5)+ ALCL cases have a good prognosis with appropriate treatment. |
| Databáze: | OpenAIRE |
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