The glycosylation profile of interleukin-2 activated human lymphocytes correlates to their anti-tumor activity
| Autor: | H, Voshol, H F, Dullens, W, Den Otter, J F, Vliegenthart |
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| Rok vydání: | 1996 |
| Předmět: |
Cytotoxicity
Immunologic 1-Deoxynojirimycin Acetylgalactosamine Glycosylation Swainsonine Lymphocyte Activation Burkitt Lymphoma Immunotherapy Adoptive Stimulation Chemical Killer Cells Natural Benzyl Compounds Carbohydrate Metabolism Humans Interleukin-2 Leukemia Erythroblastic Acute Enzyme Inhibitors Killer Cells Lymphokine-Activated Cells Cultured |
| Zdroj: | Anticancer research. 16(1) |
| ISSN: | 0250-7005 |
| Popis: | Natural killer cells display spontaneous, non-MHC-restricted cytotoxicity against tumour cells, which is strongly enhanced after incubation with IL-2. The molecular background of the increased anti-tumour activity of these lymphokine-activated killer cells is still only partly understood.In this paper, investigation has been made of the correlation between cell surface glycosylation and anti-tumour activity of LAK cells by stimulating peripheral blood lymphocytes with interleukin-2, in the presence of inhibitors of N- and O-glycosylation.Inhibition of N- or O-glycosylation of proteins during IL-2 activation leads to a 70-80% decrease in the cytolytic activity of LAK cells against K562 and Daudi tumour cells, coinciding with drastic alterations in their cell surface carbohydrate profile.The conclusion is drawn that there is a clear correlation between the glycosylation of LAK cell glycoproteins and their anti-tumour activity which points to the involvement of cell surface glycoconjugates in the development of LAK activity. |
| Databáze: | OpenAIRE |
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