A chloroplast transcript lacking the 3' inverted repeat is degraded by 3'--5' exoribonuclease activity
| ISSN: | 1355-8382 |
|---|---|
| Přístupová URL adresa: | https://explore.openaire.eu/search/publication?articleId=pmid________::29db8fb42875b13253172f3ec28278c5 https://pubmed.ncbi.nlm.nih.gov/8756408 |
| Rights: | OPEN |
| Přírůstkové číslo: | edsair.pmid..........29db8fb42875b13253172f3ec28278c5 |
| Autor: | R G, Drager, M, Zeidler, C L, Simpson, D B, Stern |
| Rok vydání: | 1996 |
| Předmět: |
Adenosine Triphosphatases
Chloroplasts Base Sequence Guanosine Models Genetic Transcription Genetic Molecular Sequence Data Polydeoxyribonucleotides Transformation Genetic Exoribonucleases Escherichia coli Animals Photosynthesis RNA Processing Post-Transcriptional Chlamydomonas reinhardtii Plasmids Repetitive Sequences Nucleic Acid Research Article |
| Zdroj: | RNA (New York, N.Y.). 2(7) |
| ISSN: | 1355-8382 |
| Popis: | Chlamydomonas reinhardtii strains harboring deletions of the chloroplast atpB 3' inverted repeat (IR) are weakly phototrophic due to reduced accumulation of discrete atpB transcripts and the chloroplast ATPase beta-subunit protein. A sequence of 18 guanosine residues, which can impede a 3'-->5' exoribonuclease in vitro, is able to substitute for the atpB IR in vivo. Strains containing the poly-guanosine tract in place of the atpB 3' IR are phototrophic and accumulate near wild-type levels of discrete atpB transcripts and the ATPase beta-subunit protein. Because these atpB transcripts contain the 18 guanosine residues, and the poly-guanosine tract is not a terminator of transcription, the accumulation of discrete atpB transcripts is likely the result of impediment of 3'-->5' exoribonuclease activity. These findings support a model in which atpB transcripts lacking the 3' IR are degraded by 3'-->5' exoribonuclease activity, and demonstrate that the poly-guanosine tract can be used to study chloroplast RNA metabolism in vivo. |
| Databáze: | OpenAIRE |
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