MicroRNA-29b sensitizes osteosarcoma cells to doxorubicin by targeting matrix metalloproteinase 9 (MMP-9) in osteosarcoma
| Autor: | D-J, Luo, L-J, Li, H-F, Huo, X-Q, Liu, H-W, Cui, D-M, Jiang |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Adult
Male Osteosarcoma Antibiotics Antineoplastic Base Sequence Antagomirs Apoptosis Bone Neoplasms Gene Expression Regulation Neoplastic MicroRNAs Young Adult Matrix Metalloproteinase 9 Cell Movement Doxorubicin Cell Line Tumor Humans Female 5' Untranslated Regions Sequence Alignment Cell Proliferation |
| Zdroj: | European review for medical and pharmacological sciences. 23(4) |
| ISSN: | 2284-0729 |
| Popis: | To discover the effect and mechanism of exogenous microRNA-29b (miR-29b) on proliferation, apoptosis and the sensitivity to chemotherapy of osteosarcoma (OS) cells.We assessed the expression of microRNA-29b in osteosarcoma tissues evaluating the regulation of in on the OS cell growth and drug sensitivity in human osteosarcoma MG-63 cell model. Firstly, quantitative RT-PCR (reverse transcription-PCR, RT-PCR) was used to measure the expression of miR-29b and matrix metallopeptidase 9(MMP-9) in primary osteosarcoma samples, and to evaluate the correlation between the two molecules. Secondly, miR-29b mimics or mimics were used to modify its expression in MG-63 cells. Luciferase reporter assay, Western blotting, cell viability, colony forming assay and apoptosis examination were performed to assess the regulation by manipulated miR-29b in the osteosarcoma-derived cells.We found that miR-29b is down-expressed, whereas the MMP-9 level was markedly higher in primary osteosarcoma tissues and osteosarcoma-derived cells. We also found that exogenous miR-29b reduces the proliferation, promotes the apoptosis and upregulates the sensitivity to chemotherapy (doxorubicin) of osteosarcoma cells via direct targeting of the MMP-9.Our data suggest that the reduced miRNA-29b may serve as a predictor of response to chemotherapy and as a therapeutic target in human osteosarcomas. |
| Databáze: | OpenAIRE |
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