Safety and efficacy of CpG-containing oligodeoxynucleotides as immunological adjuvants in rabbits

Autor:	X P, Ioannou, S M, Gomis, R, Hecker, L A, Babiuk, S, van Drunen Littel-van den Hurk
Rok vydání:	2003
Předmět:	Adjuvants Immunologic Oligonucleotides Alum Compounds Animals Cattle CpG Islands Enzyme-Linked Immunosorbent Assay Respiratory Syncytial Virus Bovine Rabbits Antibodies Viral Herpesvirus 1 Bovine
Zdroj:	Vaccine. 21(27-30)
ISSN:	0264-410X
Popis:	The efficacy of oligodeoxynucleotides containing unmethylated CpG dinucleotides (CpG ODN) as an adjuvant for rabbits was assessed alone and in combination with aluminum hydroxide (CpG/alum). The CpG/alum combination elicited a greater immune response to several antigens compared to Freund's adjuvant. A non-CpG/alum combination did not have the same effects as CpG/alum suggesting that the adjuvanticity was related to the CpG motifs. In addition, we formulated one of the antigens with combinations of CpG ODN and 30 or 10% Emulsigen (Em) [CpG/Em (30%) and CpG/Em (10%)]. Both CpG/Em (30%) and CpG/Em (10%) were more effective than Em, and equivalent to CpG/alum. The CpG/Em (10%) combination caused minimal tissue damage. Our results demonstrate that the addition of CpG ODN to aluminum hydroxide or to 10% Em significantly improves the efficiency of these adjuvants, without enhancing tissue reaction.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::295f6c35f06275ee322feb551e914d08 https://pubmed.ncbi.nlm.nih.gov/14505919 Zobrazit plný text záznamu Full Text from ScienceDirect