| Autor: |
T V, Morgan, J, McCracken, W H, Orme-Johnson, W B, Mims, L E, Mortenson, J, Peisach |
| Rok vydání: |
1990 |
| Předmět: |
|
| Zdroj: |
Biochemistry. 29(12) |
| ISSN: |
0006-2960 |
| Popis: |
Mg-ATP binds to the iron protein component of nitrogenase. The magnetic field dependence of the linear electric field effect (LEFE) in pulsed EPR is consistent with a single 4Fe-4S cluster. The LEFE is virtually unaltered when Mg-ATP is bound. Electron spin echo envelope modulation techniques were employed to evaluate the possibility of a magnetic interaction between 31P of Mg-ATP and the Fe-S center of the iron protein. None was detected. However, weak modulations possibly attributable to peptide 14N were seen, and these were slightly shifted by Mg-ATP addition. Further, protons in the vicinity of the Fe-S cluster of the protein readily exchange with D2O, and this process is unaffected by Mg-ATP. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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