Effects of busulfan on the DNA of HL-60 cells as a drug sensitivity assay

Autor:	J, Epstein, R Y, Kirdani
Rok vydání:	1986
Předmět:	Colony-Forming Units Assay Leukemia Myeloid Acute Humans DNA Neoplasm In Vitro Techniques Busulfan Cell Division Tumor Stem Cell Assay
Zdroj:	Journal of medicine. 17(1)
ISSN:	0025-7850
Popis:	We evaluated the formation of DNA-DNA cross links by busulfan as an assay for determining the sensitivity of cells to the drug. HL-60 cells were incubated with busulfan and the effects of the drug on cell growth were compared with the effects of the drug on the alkaline elution pattern of the DNA. While incubation with 100 micrograms/ml for 1 h inhibited cell growth by 50%, drug concentrations of up to 500 micrograms/ml had no effect on the elution pattern of the DNA.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::293d2c1200009fa04ff6c08e13d6f63c https://pubmed.ncbi.nlm.nih.gov/3473156 Zobrazit plný text záznamu