[Gene cloning and prokaryotic expression of glycosyltransferase from Ligustrum quihoui]

Autor: Bi-Xia, Wang, De-Hong, Xu, Chao-Yang, Tan, Ling-Min, Jiang, Yue-Fang, Luo, Lei, Meng
Rok vydání: 2017
Předmět:
Zdroj: Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica. 43(4)
ISSN: 1001-5302
Popis: According to the previous results from transcriptome analysis of Ligustrum quihoui, a glycosyltransferase gene(xynzUGT) was cloned by rapid amplification of cDNA ends(RACE). The full length cDNA of xynzUGT was 1 598 bp, consisting of 66 bp 5'-UTR, 1 440 bp ORF and 92 bp 3'-UTR. The ORF encoded a 480 amino-acid protein(xynzUGT) with a molecular weight of 54 826.67 Da and isoelectric point of 5.82. The structure of enzyme was analyzed by using bioinformatics method, the results showed that the primary structure contained a highly conserved PSPG box of glycosyltransferase, the secondary structure included α helix(38%)
Databáze: OpenAIRE