Detection and quantification of mast cell, vascular endothelial growth factor, and microvessel density in human inflammatory periapical cysts and granulomas

Autor:	T, Fonseca-Silva, C C O, Santos, L R, Alves, L C, Dias, M, Brito, A M B, De Paula, A L S, Guimarães
Rok vydání:	2012
Předmět:	Adult Male Vascular Endothelial Growth Factor A Radicular Cyst Cell Count Immunohistochemistry Epithelium Capillaries Platelet Endothelial Cell Adhesion Molecule-1 Cross-Sectional Studies Connective Tissue Microvessels Humans Female Mast Cells Tolonium Chloride Coloring Agents Periapical Granuloma
Zdroj:	International endodontic journal. 45(9)
ISSN:	1365-2591
Popis:	To identify and quantify mast cell (MC), vascular endothelial growth factor (VEGF) expression and microvessel density (MVD) in human periapical cysts and granulomas.Archived samples of cysts (n = 40) and granulomas (n = 28) were sectioned and stained with toluidine blue. MCs were identified and counted. Immunohistochemical reactions were employed to evaluate the tissue expression of VEGF and vessels. MVD was estimated by determining the areas of tissue labelled with CD31 antibody. The data were analysed using the Mann-Whitney test (P0.05).MCs were observed in the peripheral regions of both lesion types, whilst VEGF and MVD were distributed in the stroma. The presence of MCs was higher in cysts than in granulomas (P0.05). VEGF and MVD expression were similar in these lesions.The highest number of MCs was observed in cysts. Moreover, the identification of VEGF and MVD was consistent with the immune mechanisms involved in the lesions.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::285d2723634121d526fb48442bfac77c https://pubmed.ncbi.nlm.nih.gov/22486765 Zobrazit plný text záznamu Plný text