| Autor: |
Guo-Dong, Ren, Ai-Ling, Guo, Fang, Geng, Mei-Hu, Ma, Qun, Huang, Xiao-Fen, Wu |
| Rok vydání: |
2012 |
| Předmět: |
|
| Zdroj: |
Guang pu xue yu guang pu fen xi = Guang pu. 32(7) |
| ISSN: |
1000-0593 |
| Popis: |
The conformation changes of Apo-Ovotransferrin and Holo-Ovotransferrin were studied with the heat treatment 25-95 degrees C by using Fourier transform infrared spectroscopy (FTIR) and two-dimensional correlation spectroscopy analyzer. The results of one-dimensional infrared spectroscopy showed that with the increase in temperature, the peak at 3 300 cm(-1) of Apo-Ovo-transferrin shifted more than that of Holo-Ovotransferrin. The peak at 3 300 cm(-1) derived from stretching vibrations of N-H and O-H indicates that iron-binding enhanced the role of hydrogen bonds and resistance to heat. The changing order of the secondary structure of ovotransferrin was determined by analyzing two-dimensional infrared spectra,witch is beta-sheetamide II-CH2 - bending vibration. In addition, it was found that the cross-peaks at 1 652 and 1 688 cm(-1) are different in synchronous and asynchronous counter maps by comparing Apo-Ovotransferrin with Holo-Ovotransferrin. It was suggested that the temperature made less impact on the alpha-helix in Holo-Ovotransferrin than on that in Apo-Ovotransferrin, however, the beta-turn in Holo-Ovotransferrin was more sensitive to temperature. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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