| Autor: |
Baojing, Lu, Qi, Tang, Qianyun, Wang, Xuejuan, Liu, Hui, Peng, Binbin, Zhu, Li, Xie, Zeng, Li, Hanzhong, Wang, Zhenhua, Zheng, Linding, Wang, Bao, Li |
| Rok vydání: |
2021 |
| Zdroj: |
Frontiers in microbiology. 13 |
| ISSN: |
1664-302X |
| Popis: |
Enterovirus 71 (EV71) is one of the most important etiological agents for hand-foot-mouth disease. Compared with coxsackievirus A16 infection, EV71 infection is often associated with severe central nervous system complications, such as encephalitis, encephalomyelitis, and acute flaccid paralysis in infants and young children. In this study, we constructed a recombinant baculovirus with T7 ribonucleic acid polymerase under the control of a cytomegalovirus promoter and simultaneously engineered the T7 promoter upstream of a full-length EV71 complementary deoxyribonucleic acid. After transduction into mammalian cells, typical cytopathic effects (CPEs) and VP1 signals were detected in cells transfected with recombinant baculovirus. Additionally, viral particles located in the cytoplasm of human rhabdomyosarcoma cells (Rd) and Vero cells were observed by electron microscope, indicating that EV71 was recovered using a Bac-to-Bac expression system |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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