DNA Regions Responsible for Maintenance of Shingobium Plasmid pYAN-2
Autor: | Hayashi, Hiroe, Kurusu, Yasurou |
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Jazyk: | angličtina |
Rok vydání: | 2014 |
Předmět: | |
Zdroj: | Microbes and Environments |
ISSN: | 1347-4405 1342-6311 |
Popis: | We have identified and analyzed two DNA regions responsible for stable maintenance of a plasmid in the genus Sphingomonas and Escherichia coli. A 37 bp fragment, upstream of the repA gene, is required for stable maintenance of the low-copy-number small plasmid pYAN-2 (4,687 bp) from Sphingobium yanoikuyae. It does not encode any significant protein sequence and has one direct repeat for possible secondary structures. Moreover, a 70 bp fragment, upstream of the above sequence, completely stabilized the unstable pSC101 plasmid in E. coli. |
Databáze: | OpenAIRE |
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