Expression of extracellular matrix proteoglycans perlecan and decorin in carbon-tetrachloride-injured rat liver and in isolated liver cells
| Autor: | M, Gallai, I, Kovalszky, T, Knittel, K, Neubauer, T, Armbrust, G, Ramadori |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
Male
Extracellular Matrix Proteins Kupffer Cells Fibroblasts Blotting Northern Liver Cirrhosis Experimental Immunohistochemistry Extracellular Matrix Rats carbohydrates (lipids) Liver Animals Proteoglycans Endothelium Vascular Heparitin Sulfate Decorin Rats Wistar Carbon Tetrachloride Cells Cultured Heparan Sulfate Proteoglycans Research Article |
| Zdroj: | The American journal of pathology. 148(5) |
| ISSN: | 0002-9440 |
| Popis: | Proteoglycans are important components of the extracellular matrix. They are involved in liver regeneration as well as in liver fibrosis. The distribution and cellular source of proteoglycans under normal as well as pathological conditions is still under debate. Localization of decorin and perlecan was studied in normal, acutely damaged, and cirrhotic liver by histochemistry. Furthermore, their synthesis was analyzed in different liver cell populations isolated from normal rat liver. In normal liver, decorin positivity was observed in the perisinusoidal space and in the portal area. Perlecan was clearly detectable in the portal area (blood vessels and bile ducts); a moderate reaction was also seen along the sinusoids. Strong positivity for both proteoglycans was detectable in the necrotic areas of acutely damaged liver. Chronic liver damage was characterized by the deposition of decorin and perlecan in the fibrotic septa. Immunocytochemical reactions were positive for perlecan and decorin in cultured Ito and endothelial cells but not in hepatocytes and Kupffer cells. Northern hybridization confirmed the capacity of Ito cells and endothelial cells to express the two genes. Interestingly, although rat skin fibroblasts expressed strong messages for both proteoglycans, rat aortic smooth muscle cells did not synthesize decorin. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|