| Autor: |
Jing, Zhang, Jing-Zhong, Liu, Shu-Zhen, Tan, Xing-Yuan, Jia, Yan, Zhou |
| Rok vydání: |
2003 |
| Předmět: |
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| Zdroj: |
Sheng wu gong cheng xue bao = Chinese journal of biotechnology. 18(6) |
| ISSN: |
1000-3061 |
| Popis: |
To construct a new high effective genetic engineering strain which can express active PAL enzyme in Lactococcus lactis (L.L), and acquire better effect on curing hyperphenylalaninemia rats, Firstly translational fusion vector and transcriptional fusion vector were constructed in E. coli MC1061, and then PAL cDNA was transformed into L.L. Two kinds of high effect strain were compared with their enzyme activity and animal experiment was carried out. The results showed: (1) Two kinds of engineering L.L. were obtained and translational fusion strain has higher level enzyme activity. (2) The amount of transcinnamic aicd reach peak when induced for 6 hours. (3) The blood phe level of the treated rats was significantly reduced compared with non-treated rats when receiving fresh p(NZ8048-PAL)1/NZ9000. The engineering L.L(translational fusion strain) can significantly reduce the blood phe level of the hyperphenylalaninemia rats, which has more superiority than pMG36e-PAL/L. L. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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