Isolation, Culture, and Expansion of Mesenchymal Stem Cells

Autor: Izaskun, Ferrin, Izaskun, Beloqui, Lorea, Zabaleta, Juan M, Salcedo, Cesar, Trigueros, Angel G, Martin
Rok vydání: 2017
Předmět:
Zdroj: Methods in molecular biology (Clifton, N.J.). 1590
ISSN: 1940-6029
Popis: Mesenchymal stem cells (MSCs), together with hematopoietic stem cells (HSCs), are the most frequently used cell type for cell-based therapeutics. As for other cell types intended for research and translational use, it is important to establish correctly typed cell lines from human tissue donations. Here, we describe methods for isolating, culturing, and identifying MSCs from various tissues obtained through human tissue donation. The methods have been used in the context of a biobank, prepared as standard operating procedures (SOPs), ensuring traceability and reproducibility of cell production.
Databáze: OpenAIRE