Interleukin-6-producing cells in a human glioblastoma cell line are not affected by ionizing radiation

Autor:	Jean-Jacques, Dubost, Christine, Rolhion, Andrei, Tchirkov, Suzanne, Bertrand, Jacques, Chassagne, Annie, Dosgilbert, Pierre, Verrelle
Rok vydání:	2002
Předmět:	Brain Neoplasms Interleukin-6 Radiation Ionizing Tumor Cells Cultured Humans Cell Count Dose-Response Relationship Radiation Flow Cytometry Glioblastoma Cell Division
Zdroj:	Journal of neuro-oncology. 56(1)
ISSN:	0167-594X
Popis:	We investigated the production of interleukin 6 (IL-6) by a radioresistant human glioblastoma cell line G5 after single radiation events of 3, 6 and 9 Gy. The total cell number and IL-6 concentration in culture supernatant were assessed 24-96 h after irradiation. The radiation impeded or stopped G5 cell growth in a dose-dependent manner, but unexpectedly did not affect the IL-6 concentration in cell culture media that increased in the same range as in non-irradiated cultures. Furthermore, using flow cytometry, we found that the IL-6 positive cells expansion was unaffected by radiation. These findings suggested that this small (about 1%) fraction of G5 cells, constitutively producing IL-6, is highly radioresistant.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::261553dbccdeaa6b66fe97c612ebe375 https://pubmed.ncbi.nlm.nih.gov/11949824 Zobrazit plný text záznamu Full text from SpringerLink