| Autor: |
Trede, Nikolaus S., Ota, Tatsuya, Kawasaki, Hirohide, Paw, Barry H., Katz, Tammisty, Demarest, Bradley, Hutchinson, Sarah, Zhou, Yi, Hersey, Candace, Zapata, Agustin, Amemiya, Chris T, Zon, Leonard I. |
| Jazyk: |
angličtina |
| Rok vydání: |
2008 |
| Předmět: |
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| Popis: |
Generation of mature T lymphocytes requires an intact hematopoietic stem cell compartment and functional thymic epithelium. We used the zebrafish (Danio rerio) to isolate mutations that affect the earliest steps in T lymphopoiesis and thymic organogenesis. Here we describe the results of a genetic screen in which gynogenetic diploid offspring from heterozygous females were analyzed by whole mount in situ hybridization for the expression of rag-1. In order to assess immediately if a global defect in hematopoiesis resulted in the mutant phenotype, α-embryonic globin expression was simultaneously assayed for multilineage defects. In this report we present the results obtained with this strategy and show representative mutant phenotypes affecting early steps in T cell development and/or thymic epithelial cell development. We discuss the advantage of this strategy and the general usefulness of the zebrafish as a model system for vertebrate lymphopoiesis and thymic organogenesis. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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