A novel cold-inducible zinc finger protein from soybean, SCOF-1, enhances cold tolerance in transgenic plants
| Autor: | J C, Kim, S H, Lee, Y H, Cheong, C M, Yoo, S I, Lee, H J, Chun, D J, Yun, J C, Hong, S Y, Lee, C O, Lim, M J, Cho |
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| Rok vydání: | 2001 |
| Předmět: |
Cell Nucleus
Transcriptional Activation Base Sequence Sequence Homology Amino Acid Molecular Sequence Data Zinc Fingers Plants Genetically Modified Adaptation Physiological Cold Temperature Gene Expression Regulation Plant Genes Reporter Amino Acid Sequence Soybeans Heat-Shock Proteins Abscisic Acid DNA Primers Glucuronidase Plant Proteins Transcription Factors |
| Zdroj: | The Plant journal : for cell and molecular biology. 25(3) |
| ISSN: | 0960-7412 |
| Popis: | Cold stress on plants induces changes in the transcription of cold response genes. A cDNA clone encoding C2H2-type zinc finger protein, SCOF-1, was isolated from soybean. The transcription of SCOF-1 is specifically induced by low temperature and abscisic acid (ABA) but not by dehydration or high salinity. Constitutive overexpression of SCOF-1 induced cold-regulated (COR) gene expression and enhanced cold tolerance of non-acclimated transgenic Arabidopsis and tobacco plants. SCOF-1 localized to the nucleus but did not bind directly to either C-repeat/dehydration (CRT/DRE) or ABA responsive element (ABRE), cis-acting DNA regulatory elements present in COR gene promoters. However, SCOF-1 greatly enhanced the DNA binding activity of SGBF-1, a soybean G-box binding bZIP transcription factor, to ABRE in vitro. SCOF-1 also interacted with SGBF-1 in a yeast two-hybrid system. The SGBF-1 transactivated the beta-glucuronidase reporter gene driven by the ABRE element in Arabidopsis leaf protoplasts. Furthermore, the SCOF-1 enhanced ABRE-dependent gene expression mediated by SGBF-1. These results suggest that SCOF-1 may function as a positive regulator of COR gene expression mediated by ABRE via protein-protein interaction, which in turn enhances cold tolerance of plants. |
| Databáze: | OpenAIRE |
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