| Autor: |
J F, Borrego, M K, Huelsmeyer, M E, Pinkerton, J L, Muszynski, S A K, Miller, I D, Kurzman, D M, Vail |
| Rok vydání: |
2013 |
| Předmět: |
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| Zdroj: |
Veterinary and comparative oncology. 14(2) |
| ISSN: |
1476-5829 |
| Popis: |
We interrogated the neurokinin-1 receptor (NK-1R)/substance P (SP) pathway in canine melanoma tumour tissues and cell lines. NK-1R messenger RNA (mRNA) and protein expression were observed in the majority of tumour tissues. Immunohistochemical assessment of archived tissue sections revealed NK-1R immunoreactivity in 11 of 15 tumours, which may have diagnostic, prognostic and therapeutic utility. However, we were unable to identify a preclinical in vitro cell line or in vivo xenograft model that recapitulates NK-1R mRNA and protein expression documented in primary tumours. While maropitant inhibited proliferation and enhanced apoptosis in cell lines, in the absence of documented NK-1R expression, this may represent off-target effects. Furthermore, maropitant failed to suppress tumour growth in a canine mouse xenograft model derived from a cell line expressing mRNA but not protein. While NK-1R represents a novel target, in the absence of preclinical models, in-species clinical trials will be necessary to investigate the therapeutic potential for antagonists such as maropitant. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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