Enantioselective determination of isradipine in human serum using chiral stationary phase liquid chromatography and gas chromatography with nitrogen selective detection

Autor:	H S, Rask, H R, Angelo, H R, Christensen
Rok vydání:	1998
Předmět:	Chromatography Gas Humans Stereoisomerism Isradipine Calcium Channel Blockers Chromatography High Pressure Liquid
Zdroj:	Chirality. 10(9)
ISSN:	0899-0042
Popis:	rac-Isradipine is a dihydropyridine type calcium antagonist. Its calcium entry blocking effect is due primarily to the (+)-(S)-enantiomer. This study describes a sensitive enantioselective method for the determination of isradipine in human serum. Following alkaline extraction into hexane, the enantiomers of isradipine are separated quantitatively by high-performance liquid chromatography on a Chiralcel OJ column at 39 degrees C. The collected fractions were evaporated and assayed using capillary gas chromatography on a HP 50+ column with nitrogen selective detection. Using 2.0 ml of serum, 0.7 nmol/1 (0.26 ng/ml) of each enantiomer could be determined with acceptable precision. The method has successfully been used to measure (+)-(S)- and (-)-(R)-isradipine concentrations in samples from volunteers after intravenous and oral administration of isradipine.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::25ad35db9d576ae15b056174874ad6f2 https://pubmed.ncbi.nlm.nih.gov/9839430 Zobrazit plný text záznamu Plný text