| Autor: |
M E, Ressing, W J, van Driel, E, Celis, A, Sette, M P, Brandt, M, Hartman, J D, Anholts, G M, Schreuder, W B, ter Harmsel, G J, Fleuren, B J, Trimbos, W M, Kast, C J, Melief |
| Rok vydání: |
1996 |
| Předmět: |
|
| Zdroj: |
Cancer research. 56(3) |
| ISSN: |
0008-5472 |
| Popis: |
Most cervical carcinoma (Cxca) cells constitutively express human papillomavirus type 16 (HPV16) E6 and E7 oncoproteins. These proteins are, therefore, attractive targets for T cell-based immunotherapy. Previously, we identified HVP16 E7-encoded CTL epitopes. In patients with cervical intraepithelial neoplasia or Cxca, little is known concerning T-cell activity against viruses in general and against HPV16 in particular. Here, we have screened the blood of 10 healthy donor controls and of 22 patients with HPV16+ cervical lesions for the presence of CTLs directed against HPV16 E7- and control influenza virus matrix-derived epitopes presented by HLA-A *0201. We detected influenza virus-specific CTLs in all donors and in the majority of patients, indicating that most patients have functioning T-cell responses despite their lesions or therapeutic interventions. Moreover, we show that patients with HPV16+ lesions occasionally have memory CTLs against a HPV16 E7-encoded epitope (sequence YMLD-LQPETT), providing evidence for natural CTL immunity against HPV16 in patients with cervical lesions. Combined, these findings raise possibilities for vaccination with HPV16 E7-encoded peptides to induce or augment CTL responses for treatment or prevention of Cxca. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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