| Autor: |
O T, Samko, A A, Kalugin, M A, El'darov, I V, Karpychev, N V, Anikeĭcheva, E B, Khoroshutina, K G, Skriabin, G I, Librik, N N, Sokolov |
| Rok vydání: |
1994 |
| Předmět: |
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| Zdroj: |
Bioorganicheskaia khimiia. 20(12) |
| ISSN: |
0132-3423 |
| Popis: |
New site-specific endonucleases BciBI and BciBII have been detected in Bacillus circulans. The enzymes were purified by fractionation of cell-free extract with polyethylene imine and ammonium sulphate (40-80% of saturation) followed by chromatography on DEAE-sepharose, blue-sepharose and phosphocellulose. The endonucleases BciBI and BciBII were separated only at the final step of the purification--by chromatography on the phosphocellulose column. The yields of BciBI and BciBII were 600 and 10,000 U/g of cells. It was found that restriction endonucleases BciBI and BciBII are isoschizomers of ClaI and BstNI, respectively. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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