| Autor: |
Dibyajnan, Chakraborty, Sagarmoy, Ghosh |
| Rok vydání: |
2020 |
| Předmět: |
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| Zdroj: |
Methods in molecular biology (Clifton, N.J.). 2167 |
| ISSN: |
1940-6029 |
| Popis: |
The epsilon domain of Hepatitis B virus plays a crucial role in encapsidation of viral pregenomic RNA and its partial NMR structure has been determined. However, we recently described a potassium-dependent ribonucleolytic activity associated with this region, so that a 53 nt long RNA containing the epsilon domain could release itself and cleaved other RNAs. We describe here the experimental methodologies for setting up the reactions and outline a general strategy for initial demonstration of this self-cleaving ribozyme activity. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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