| Autor: |
Lisa, Ufarté, Sophie, Bozonnet, Elisabeth, Laville, Davide A, Cecchini, Sandra, Pizzut-Serin, Samuel, Jacquiod, Sandrine, Demanèche, Pascal, Simonet, Laure, Franqueville, Gabrielle Potocki, Veronese |
| Rok vydání: |
2016 |
| Předmět: |
|
| Zdroj: |
Methods in molecular biology (Clifton, N.J.). 1399 |
| ISSN: |
1940-6029 |
| Popis: |
Activity-based metagenomics is one of the most efficient approaches to boost the discovery of novel biocatalysts from the huge reservoir of uncultivated bacteria. In this chapter, we describe a highly generic procedure of metagenomic library construction and high-throughput screening for carbohydrate-active enzymes. Applicable to any bacterial ecosystem, it enables the swift identification of functional enzymes that are highly efficient, alone or acting in synergy, to break down polysaccharides and oligosaccharides. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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