| Autor: |
O A, Vinogradova, V G, Savchenko, E V, Domracheva, L S, Liubimova, Iu V, Ol'shanskaia, L V, Diachenko, E N, Parovichnikova, L P, Mendeleeva, A V, Zakharova |
| Rok vydání: |
2002 |
| Předmět: |
|
| Zdroj: |
Terapevticheskii arkhiv. 74(7) |
| ISSN: |
0040-3660 |
| Popis: |
To study trends in restoration of normal and tumor hemopoiesis after transplantation of allogenic and syngenic hemopoietic cells.The examination of bone marrow before transplantation and 1, 2, 3, 6, 9 months, 1, 2 and 3 years after bone marrow transplantation (BMT) was made in 25 patients with chronic myeloid leukemia (CML) after allogenic transplantation of the bone marrow (TBM) and 4 patients after syngenic TBM. The method of G-differential staining of chromosomes and fluorescent hybridization in situ (FISH) with DNA probe to centromeric sites X/Y of chromosomes and genes BCR/ABL was used.56% of CML patients after allogenic BMT were in cytogenetic and clinicohematological remission, 16% developed early cytogenetic recurrence. Single metaphase with t(9;22) were identified in 28%; 14.3% developed late cytogenetic and hematological recurrence. In patients in posttransplantation remission there were from 0.1 to 5.8% cells of the host. The number of cells of the host and the number of BCR/ABL-positive cells correlated significantly.The results of 8-year monitoring of chimerism and minimal residual disease validate application of molecular-cytogenetic methods for assessing transplant condition. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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