| Autor: |
M M, Blanchard, V, Nowotny |
| Rok vydání: |
1994 |
| Předmět: |
|
| Zdroj: |
Genetic analysis, techniques and applications. 11(1) |
| ISSN: |
1050-3862 |
| Popis: |
An automation-assisted method enables DNA extraction of over a thousand yeast colonies in a day by one person. Yeast DNA is extracted essentially quantitatively in three steps: (a) cells are converted into spheroplasts with yeast lytic enzyme, (b) cells are lysed with proteinase K, and (c) DNA is collected by ethanol precipitation. The DNA stored at 4 degrees C is active in polymerase chain reaction experiments for more than a year. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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