Isolation and characterization of all-trans-retinoic acid-responsive genes in the rat testis

Autor:	I C, Gaemers, A M, Van Pelt, A P, Themmen, D G, De Rooij
Rok vydání:	1998
Předmět:	Male Genomic Library DNA Complementary Vitamin A Deficiency Tretinoin Rats Electron Transport Complex IV Clusterin Gene Expression Regulation Pregnancy Testis Animals Female Rats Wistar Spermatogenesis Glycoproteins Molecular Chaperones
Zdroj:	Molecular reproduction and development. 50(1)
ISSN:	1040-452X
Popis:	By way of differential screening of testis cDNA libraries from vitamin A-deficient (VAD) rats before and after administration of all-trans retinoic acid (ATRA), genes, the transcription of which was influenced by ATRA, were isolated. Most clones with an increased transcription encoded different subunits of the same mitochondrial protein complex, cytochrome c oxidase (COX). The mRNA expression of COX increased by a factor 3.9 +/- 1.5 (mean +/- SD, n = 4). This increased expression seems to reflect an increased energy demand in the ATRA-supplemented VAD testis. Also, one gene was isolated, the transcription of which was reduced to about 70% by ATRA. This gene, sulfated glycoprotein 2 (Sgp-2), is a major secretion product of Sertoli cells, the function of which is still unknown. The effect of ATRA on Sgp-2 expression may be direct, since the promoter of Sgp-2 contains a putative ATRA-responsive element (RARE).
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::24860978eac4d52598bd694e5ef73be6 https://pubmed.ncbi.nlm.nih.gov/9547504 Zobrazit plný text záznamu Plný text