Encapsulation of Negatively Charged Cargo in MS2 Viral Capsids

Autor: Ioana L, Aanei, Jeff E, Glasgow, Stacy L, Capehart, Matthew B, Francis
Rok vydání: 2018
Předmět:
Zdroj: Methods in molecular biology (Clifton, N.J.). 1776
ISSN: 1940-6029
Popis: Encapsulation into virus-like particles is an efficient way of loading cargo of interest for delivery applications. Here, we describe the encapsulation of proteins with tags comprising anionic amino acids or DNA and gold nanoparticles with negative surface charges inside MS2 bacteriophage capsids to obtain homogeneous nanoparticles with a diameter of 27 nm.
Databáze: OpenAIRE