Cyclooxygenase (COX)-2 and Granulosa Cell Apoptosis In Vitro
| Autor: | Caffrey, Angela S., Patton, William C., Corselli, Johannah U., Swensen, Ron E., King, Alan, Chan, Philip J. |
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| Jazyk: | angličtina |
| Rok vydání: | 2002 |
| Předmět: |
endocrine system
Sulfonamides Granulosa Cells Cyclooxygenase 2 Inhibitors Dose-Response Relationship Drug urogenital system Cell Survival Genes myc Membrane Proteins Apoptosis Exons Oxidants Article Isoenzymes Celecoxib Cyclooxygenase 2 Prostaglandin-Endoperoxide Synthases Cyclooxygenase 1 Humans Pyrazoles Cyclooxygenase Inhibitors Female Cells Cultured |
| Popis: | C-myc was studied in cyclooxygenase (COX)-2 associated granulosa cell apoptosis,Granulosa cells (N = 5 cases) were incubated for 24 h in either 1 or 50 microM COX-2 inhibitor, 1 or 50 microM COX-1/COX-2 inhibitor, negative or positive controls Single primer polymerase chain reaction of c-myc exon 1 were performed. Bisbenzimide-stained control single-stranded (ssDNA) were hybridized to SYBR Gold-stained ssDNA and fluorescent images analyzed.C-myc was disrupted by the high-dose COX-2 inhibitor. Cell viability decreased with COX-1 and COX-2 inhibition. However, cell viability was similar for the positive control and at low-dose COX-2 inhibition.Inhibition of both COX-1 and COX-2 initiated apoptosis without disrupting c-myc suggesting a protective effect on c-myc. The low dosage of the COX-2 inhibitor did not disrupt c-myc and cell viability. C-myc sensitization was not part of apoptosis. |
| Databáze: | OpenAIRE |
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