In Vivo Analysis of Glial Immune Responses to Axon Degeneration in Drosophila melanogaster
| Autor: | Mary A, Logan, Sean D, Speese |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Central Nervous System
DNA Complementary Microscopy Confocal Intravital Microscopy Axotomy Nerve Tissue Proteins Immunohistochemistry Polymerase Chain Reaction Axons Drosophila melanogaster Gene Expression Regulation Phagocytosis Genes Reporter Image Processing Computer-Assisted Animals Drosophila Proteins Wallerian Degeneration Neuroglia |
| Zdroj: | Methods in molecular biology (Clifton, N.J.). 2143 |
| ISSN: | 1940-6029 |
| Popis: | Axon degeneration elicits a range of immune responses from local glial cells, including striking changes in glial gene expression, morphology, and phagocytic activity. Here, we describe a detailed set of protocols to assess discrete components of the glial reaction to axotomy in the adult nervous system of Drosophila melanogaster. These methods allow one to visualize and quantify transcriptional, morphological, and functional responses of glia to degenerating axons in a model system that is highly amenable to genetic manipulation. |
| Databáze: | OpenAIRE |
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