Counter-regulatory responses to
| Autor: | Toyin Mohammed, Salman, Isiaka Abdullateef, Alagbonsi, Sheu Oluwadare, Sulaiman |
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| Rok vydání: | 2020 |
| Předmět: |
Plasma glucose
LDH Lactate dehydrogenase ANOVA Analysis of Variance G6P Glucose-6-phosphate Telfairia occidentalis SEM Standard error of mean Counter-regulatory hormones LSD Least Significance Difference NAD Nicotinamide adenine dinucleotide G6Pase Glucose-6-phosphatase Original Research Paper Glucoregulatory enzymes GCK Glucokinase TO Telfairia occidentalis NIH National Institutes of Health ELISA Enzyme-linked immunosorbent assay Avidin-HRP Avidin-Horseradish Peroxidase EGP Endogenous glucose production Insulin G6PD Glucose-6-phosphate dehydrogenase SPSS Statistical Package for the Social Sciences cAMP Cyclic adenosine monophosphate |
| Zdroj: | Metabolism Open |
| ISSN: | 2589-9368 |
| Popis: | Background Telfairia occidentalis (TO) has many biological activities including blood glucose regulation. Thus, it is being used in the treatment of diabetes mellitus. TO has been shown to cause insulin-mediated hypoglycaemia, which leads to post-hypoglycaemic hyperglycaemia. However, the mechanism involved in the post-hypoglycaemic hyperglycaemia is still poorly understood. Objective This research was designed to determine the response of glucoregulatory hormones and enzymes to TO treatment. Methods Thirty-five male Wistar rats were divided into seven oral treatment groups (n = 5/group), which received either of 100 mg/kg or 200 mg/kg TO for 7-, 10- or 14 days. Results The 7-day treatment with TO significantly increased the levels of insulin, glucagon, and glucose-6-phosphatase (G6Pase) activity but decreased the levels of glucose, adrenaline, and glucokinase (GCK) activity. The 10-day treatment with 100 mg/kg TO increased glucose and decreased GCK activity while 200 mg/kg for the same duration increased glucose, insulin, GCK and G6Pase activities but reduced glucagon. The 14-day treatment with 100 mg/kg TO decreased glucose and glucagon but increased cortisol, while 200 mg/kg TO for same duration increased insulin, but reduced glucagon and GCK activity. Conclusion The TO’s post-hypoglycaemic hyperglycaemia results from increased glucagon and G6Pase activity, and reduced GCK activity. Moreover, the glucagon response mainly depends on glucose rather than insulin. |
| Databáze: | OpenAIRE |
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