The tissue culture and morphology of human breast tumor cell line BOT-2

Autor: R E, Nordquist, D R, Ishmael, C A, Lovig, D M, Hyder, A F, Hoge
Rok vydání: 1975
Předmět:
Zdroj: Cancer research. 35(11 Pt 1)
ISSN: 0008-5472
Popis: A continuous human breast tumor cell line (BOT-2) was derived from an infiltrating duct carcinoma. The tumor cell line was grown as a monolayer in flasks, but the cells could be readily adapted to growth in roller cultures. These studies indicate that BOT-2 cells have a 16- to 18-hr doubling time and a modal chromosomal number of 63. The original BOT-2 cell culture has been in continuous cultivation for almost 2 years and has been passed 137 times. The BOT-2 cell line has been differentiated from HeLa cells by isoenzyme studies and chromosomal analysis.
Databáze: OpenAIRE