Glucose-6-phosphate dehydrogenase from a tetracycline producing strain of Streptomyces aureofaciens: some properties and regulatory aspects of the enzyme

Autor:	J, Neuzil, J, Novotná, V, Erban, V, Bĕhal, Z, Hostálek
Rok vydání:	1988
Předmět:	Enzyme Activation Kinetics Cations Divalent Streptomyces aureofaciens Glucosephosphate Dehydrogenase Hydrogen-Ion Concentration Tetracycline NAD NADP
Zdroj:	Biochemistry international. 17(1)
ISSN:	0158-5231
Popis:	Glucose-6-phosphate dehydrogenase from Streptomyces aureofaciens exhibited activity with both NAD and NADP, the maximum reaction rate being 1.6 times higher for NAD-linked activity than for the NADP-linked one. The KM values for NAD-linked activity were 2.5 mM for glucose-6-phosphate and 0.27 mM for NAD, and for NADP-linked activity 0.8 mM for glucose-6-phosphate and 0.08 mM for NADP. NAD- and NADP-linked activities were inhibited by both NADH and NADPH. (2'-phospho-)adenosinediphospho-ribose inhibited only NAD-linked activity. The inhibition was competitive with respect to NAD and noncompetitive with respect to glucose-6-phosphate.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::22be2ab3ddc87ca1c36a7ea94f5d58c4 https://pubmed.ncbi.nlm.nih.gov/3142475 Zobrazit plný text záznamu