Attenuation of Experimental Autoimmune Hepatitis in Mice with Bone Mesenchymal Stem Cell-Derived Exosomes Carrying MicroRNA-223-3p
| Autor: | Lu, Feng-Bin, Chen, Da-Zhi, Chen, Lu, Hu, En-De, Wu, Jin-Lu, Li, Hui, Gong, Yue-Wen, Lin, Zhuo, Wang, Xiao-Dong, Li, Ji, Jin, Xiao-Ya, Xu, Lan-Man, Chen, Yong-Ping |
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| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Male
STAT3 Transcription Factor Macrophages autoimmune liver disease Mesenchymal Stem Cells Articles exosomes Immunomodulation Mice Inbred C57BL Disease Models Animal Hepatitis Autoimmune Mice MicroRNAs RAW 264.7 Cells Liver Animals Cytokines mesenchymal stromal cells immunomodulatory Cells Cultured |
| Zdroj: | Molecules and Cells |
| ISSN: | 0219-1032 1016-8478 |
| Popis: | MicroRNA-223-3p (miR-223-3p) is one of the potential microRNAs that have been shown to alleviate inflammatory responses in pre-clinical investigations and is highly encased in exosomes derived from bone mesenchymal stem cells (MSC-exosomes). MSC-exosomes are able to function as carriers to deliver microRNAs into cells. Autoimmune hepatitis is one of the challenging liver diseases with no effective treatment other than steroid hormones. Here, we examined whether MSC-exosomes can transfer miR-223-3p to treat autoimmune hepatitis in an experimental model. We found that MSC-exosomes were successfully incorporated with miR-223-3p and delivered miR-223-3p into macrophages. Moreover, there was no toxic effect of exosomes on the macrophages. Furthermore, treatments of either exosomes or exosomes with miR-223-3p successfully attenuated inflammatory responses in the liver of autoimmune hepatitis and inflammatory cytokine release in both the liver and macrophages. The mechanism may be related to the regulation of miR-223-3p level and STAT3 expression in the liver and macrophages. These results suggest that MSC-exosomes can be used to deliver miR-223-3p for the treatment of autoimmune hepatitis. |
| Databáze: | OpenAIRE |
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