Improved lectin agglutination method for T-cell depletion of HLA-mismatched bone marrow grafts in children

Autor:	T, Schreiner, M, Wiesneth, I, Slaper-Cortenbach, B, Maccari, E, Erne, C, Bischof, S, Müller, W, Friedrich, B, Kubanek
Rok vydání:	1996
Předmět:	Agglutination Child Preschool Histocompatibility Testing Lectins Graft vs Host Disease Humans Transplantation Homologous Child Lymphocyte Depletion Bone Marrow Transplantation
Zdroj:	Transfusion science. 17(4)
ISSN:	0955-3886
Popis:	For T-cell depletion in HLA-nonidentical bone marrow transplantation of children with malignant diseases, we improved the original lectin/rosetting method described in 1981 by adding anti-CD2/3 coated donor red blood cells to the combination to achieve lectin agglutination in one step. Further improvements in handling led to a shortened and simplified method and better quality of the graft. Five bone marrow grafts prepared with this modified protocol contained a median number of 6 (0-28) x 10(4) T-cells per kg, corresponding to 0.02 (0-0.08)% CD3+ cells and 6 (3.7-10.5) x 10(6) CD34+ cells per kg at a median body-weight of 7 (5-38)kg. The overall recoveries after T-cell depletion were: NC 17 (10-44)%, CD34+ cells 61 (22-100)%, and CFU-GM 55 (29-212)%.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::2290bb511fd694b5695ac3b9692959a8 https://pubmed.ncbi.nlm.nih.gov/10168564 Zobrazit plný text záznamu Full Text from ScienceDirect