Use of multiple peptides containing T cell epitopes is a feasible approach for peptide-based immunotherapy in Can f 1 allergy
| Autor: | Anu K, Immonen, Antti H, Taivainen, Ale T O, Närvänen, Tuure T, Kinnunen, Soili A, Saarelainen, Marja A, Rytkönen-Nissinen, Tuomas I, Virtanen |
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| Rok vydání: | 2007 |
| Předmět: |
T-Lymphocytes
fungi Dose-Response Relationship Immunologic food and beverages Epitopes T-Lymphocyte Original Articles Allergens Antigens Plant Lymphocyte Activation Peptide Fragments Cell Line Immunophenotyping Interleukin-10 Interferon-gamma Dogs Hypersensitivity Animals Cytokines Feasibility Studies Humans Immunotherapy Interleukin-4 Cells Cultured Cell Proliferation |
| Zdroj: | Immunology. 120(1) |
| ISSN: | 0019-2805 |
| Popis: | We have previously shown that the major dog allergen Can f 1 contains seven T cell epitope regions, none of which was preferentially recognized. To identify the immune characteristics of Can f 1 epitopes and to verify their suitability for peptide-based allergen immunotherapy, short-term T cell lines were generated with epitope-containing peptides from peripheral blood mononuclear cells of Can f 1 skinprick test-positive allergic and healthy control subjects. The lines were examined for their proliferative capacity and cytokine production upon stimulation with the allergen peptide, a homologous peptide from human tear lipocalin (TL) and Can f 1 and TL proteins. Can f 1 peptides induced proliferation of T cells and gave rise to T cell lines with comparable efficiencies. In particular, the T cell lines of allergic subjects induced with p33-48 and p107-122 favoured the production of interferon-gamma and interleukin-10, respectively. A greater number of Can f 1-specific T cell lines were generated from allergic than from healthy individuals. Two p107-122-induced Can f 1-specific T cell lines also reacted to a homologous peptide of human TL. Our results suggest that several T cell epitope-containing peptides should be used in combination for specific immunotherapy in Can f 1 allergy. |
| Databáze: | OpenAIRE |
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