Identification of bacterial cell-surface virulence determinants with TnphoA

Autor: M R, Kaufman, R K, Taylor
Rok vydání: 1994
Předmět:
Zdroj: Methods in enzymology. 235
ISSN: 0076-6879
Popis: Insertion mutagenesis using TnphoA has proved to be a potent device for the creation of easily screened knockout mutations in genes encoding virulence determinants in a variety of pathogenic bacteria. Initial identification of genes with TnphoA directly initiates more sophisticated genetic and biochemical studies on these factors essential to our understanding of bacterial pathogenesis.
Databáze: OpenAIRE