Autor: |
M R, Kaufman, R K, Taylor |
Rok vydání: |
1994 |
Předmět: |
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Zdroj: |
Methods in enzymology. 235 |
ISSN: |
0076-6879 |
Popis: |
Insertion mutagenesis using TnphoA has proved to be a potent device for the creation of easily screened knockout mutations in genes encoding virulence determinants in a variety of pathogenic bacteria. Initial identification of genes with TnphoA directly initiates more sophisticated genetic and biochemical studies on these factors essential to our understanding of bacterial pathogenesis. |
Databáze: |
OpenAIRE |
Externí odkaz: |
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