| Autor: |
M V, Gorelaia, E N, Zhmareva, V A, Berezin |
| Rok vydání: |
1992 |
| Předmět: |
|
| Zdroj: |
Ukrainskii biokhimicheskii zhurnal (1. 64(5) |
| ISSN: |
0201-8470 |
| Popis: |
Phenomena of the binding of poor-soluble placenta proteins (PSPP) with pregnant women sera IgG as well as placenta blood IgG were studied. PSPP were extracted from the placenta tissue, washed out from soluble proteins, by the use of 3M KCl solution containing 0.005 M PMSF. PSPP were separated by the use of two-dimensional isoelectrofocusing and SDS-PAG electrophoresis and more than 30 different polypeptides were visualized. Having used various ELISA procedures with pregnant women sera IgG, placenta blood IgG as well as its Fab and Fc-fragments we have shown that both the receptor-type and the antigen-antibody-like interaction of PSPP took place. Both the polypeptide compositions and the isoelectrofocusing points ranges of the antigen-antibody-like interacting IgG-binding PSPP were determined by the use of the peroxidase conjugated Fab-fragments of the placenta blood IgG. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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