A selective small molecule inhibitor of c-Met kinase inhibits c-Met-dependent phenotypes in vitro and exhibits cytoreductive antitumor activity in vivo
| Autor: | James G, Christensen, Randall, Schreck, Jon, Burrows, Poonam, Kuruganti, Emily, Chan, Phuong, Le, Jeffrey, Chen, Xueyan, Wang, Lany, Ruslim, Robert, Blake, Kenneth E, Lipson, John, Ramphal, Steven, Do, Jingrong J, Cui, Julie M, Cherrington, Dirk B, Mendel |
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| Rok vydání: | 2003 |
| Předmět: |
Indoles
Lung Neoplasms Mice Nude Antineoplastic Agents Proto-Oncogene Proteins c-met Kidney Xenograft Model Antitumor Assays Cell Line Rats Pancreatic Neoplasms Kinetics Mice Dogs Stomach Neoplasms Cell Line Tumor NIH 3T3 Cells Animals Humans Female Sulfones Drug Screening Assays Antitumor Enzyme Inhibitors Phosphorylation Signal Transduction |
| Zdroj: | Cancer research. 63(21) |
| ISSN: | 0008-5472 |
| Popis: | The c-Met receptor tyrosine kinase and its ligand, hepatocyte growth factor (HGF), have been implicated in the development and progression of several human cancers and are attractive targets for cancer therapy. PHA-665752 was identified as a small molecule, ATP-competitive, active-site inhibitor of the catalytic activity of c-Met kinase (K(i) 4 nM). PHA-665752 also exhibited50-fold selectivity for c-Met compared with a panel of diverse tyrosine and serine-threonine kinases. In cellular studies, PHA-665752 potently inhibited HGF-stimulated and constitutive c-Met phosphorylation, as well as HGF and c-Met-driven phenotypes such as cell growth (proliferation and survival), cell motility, invasion, and/or morphology of a variety of tumor cells. In addition, PHA-665752 inhibited HGF-stimulated or constitutive phosphorylation of mediators of downstream signal transduction of c-Met, including Gab-1, extracellular regulated kinase, Akt, signal transducer and activator of transcription 3, phospholipase C gamma, and focal adhesion kinase, in multiple tumor cell lines in a pattern correlating to the phenotypic response of a given tumor cell. In in vivo studies, a single dose of PHA-665752 inhibited c-Met phosphorylation in tumor xenografts for up to 12 h. Inhibition of c-Met phosphorylation was associated with dose-dependent tumor growth inhibition/growth delay over a repeated administration schedule at well-tolerated doses. Interestingly, potent cytoreductive activity was demonstrated in a gastric carcinoma xenograft model. Collectively, these results demonstrate the feasibility of selectively targeting c-Met with ATP-competitive small-molecules and suggest the therapeutic potential of targeting c-Met in human cancers. |
| Databáze: | OpenAIRE |
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