| Autor: |
Xiao-Xi, Kou, Qing-Ping, Wu, Hong-Ying, Fan |
| Rok vydání: |
2006 |
| Předmět: |
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| Zdroj: |
Wei sheng wu xue bao = Acta microbiologica Sinica. 46(2) |
| ISSN: |
0001-6209 |
| Popis: |
Rotavirus is one of the major cause of the viral gastroenteritis throughout the world. A nucleic acid sequence-based amplification(NASBA) technique for the detection of rotavirus in faecal specimens was developed and compared to the RT-PCR technique. Primers were designed according to the high conserved region of VP7 gene. Amplicons were detected by denaturating agarose gel, and then demonstrated by Northern hybridization with digoxigenin-labeled oligonucleotide probe. The anticipative specific amplification product is 392bp,and no nonspecific products appear even the concentration of nontarget nucleic acid as high as 1 microg/microL. A detection limit of 50 pg target RNA/mL is obtained when the optimal amplification time of 3h used.The NASBA assay will be a favourable alternative to RT-PCR for the investigation of rotavirus outbreaks as a routine diagnostic test in the near future because it is shown to be highly sensitive, specific and do not require specialized equipment. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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