Activation of the neutrophil respiratory burst by plasma from periodontitis patients is mediated by pro-inflammatory cytokines
| Autor: | Irundika H K, Dias, John B, Matthews, Iain L C, Chapple, Helen J, Wright, Christopher R, Dunston, Helen R, Griffiths |
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| Rok vydání: | 2010 |
| Předmět: |
Adult
Male Luminescence Chemotactic Factors Interleukin-8 Granulocyte-Macrophage Colony-Stimulating Factor Interferon-alpha Middle Aged Neutrophil Activation Recombinant Proteins Statistics Nonparametric Stimulation Chemical N-Formylmethionine Leucyl-Phenylalanine Plasma Case-Control Studies Chronic Periodontitis Cytokines Humans Female Inflammation Mediators Respiratory Burst |
| Zdroj: | Journal of clinical periodontology. 38(1) |
| ISSN: | 1600-051X |
| Popis: | To determine the effect of periodontitis patients' plasma on the neutrophil oxidative burst and the role of albumin, immunoglobulins (Igs) and cytokines.Plasma was collected from chronic periodontitis patients (n=11) and periodontally healthy controls (n=11) and used with/without depletion of albumin and Ig or antibody neutralization of IL-8, GM-CSF or IFN-α to prime/stimulate peripheral blood neutrophils, isolated from healthy volunteers. The respiratory burst was measured by lucigenin-dependent chemiluminescence. Plasma cytokine levels were determined by ELISA.Plasmas from patients were significantly more effective in both directly stimulating neutrophil superoxide production and priming for subsequent formyl-met-leu-phe (fMLP)-stimulated superoxide production than plasmas from healthy controls (p0.05). This difference was maintained after depletion of albumin and Ig. Plasma from patients contained higher mean levels of IL-8, GM-CSF and IFN-α. Individual neutralizing antibodies against IL-8, GM-CSF or IFN-α inhibited the direct stimulatory effect of patients' plasma, whereas the ability to prime for fMLP-stimulated superoxide production was only inhibited by neutralization of IFN-α. The stimulating and priming effects of control plasma were unaffected by antibody neutralization.This study demonstrates that plasma cytokines may have a role in inducing the hyperactive (IL-8, GM-CSF, IFN-α) and hyper-reactive (IFN-α) neutrophil phenotype seen in periodontitis patients. |
| Databáze: | OpenAIRE |
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