| Autor: |
Dan-Lei, Mou, Ying-Peng, Wang, Hong, Jiang, Shu-Yuan, Xiao, Xu, Yu, Guang-Yu, Li, Ping-Zhong, Wang, Yong-Tao, Sun, Qing-He, Nie, Chang-Xing, Huang, Xue-Fan, Bai |
| Rok vydání: |
2007 |
| Předmět: |
|
| Zdroj: |
Bing du xue bao = Chinese journal of virology. 23(6) |
| ISSN: |
1000-8721 |
| Popis: |
The G1 cytoplasmic tail of Hantaan virus (HTNV) harbors a highly conserved region, which is homologous to immunoreceptor tyrosine-based activation motifs (ITAM) and is termed the ITAM-like sequence. To demonstrate the potential signal-transducing activity of G1 ITAM-like sequence resembling the canonical ITAM within immune and endothelial cells, a series of experiments were performed to define its interaction with cellular kinases. The synthesized G1 ITAM-like peptide was shown to coprecipitate with cellular phosphoprotein complexes by an immune-complex kinase assay. Mutational analyses showed that this ITAM-like sequence was a substrate for the Src family kinase Fyn, and two conserved tyrosine residues were required for coprecipitating Lyn, Syk, and ZAP-70 kinases. These findings demonstrated that HTNV envelope glycoprotein G1 contains a functional ITAM-like sequence in its cytoplasmic tail, which can bind critical cellular kinases that regulate immune and endothelial cell functions. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
|
