IL-6 and PPARgamma signalling in human PC-3 prostate cancer cells
| Autor: | Nea, Pitulis, Efstathia, Papageorgiou, Roxane, Tenta, Peter, Lembessis, Michael, Koutsilieris |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
Male
Mitogen-Activated Protein Kinase 1 STAT3 Transcription Factor Mitogen-Activated Protein Kinase 3 Interleukin-6 Reverse Transcriptase Polymerase Chain Reaction Blotting Western Prostatic Neoplasms PPAR gamma Tumor Cells Cultured Humans RNA Messenger Phosphorylation Proto-Oncogene Proteins c-akt Cell Proliferation Signal Transduction |
| Zdroj: | Anticancer research. 29(6) |
| ISSN: | 0250-7005 |
| Popis: | Peroxisome proliferator-activated receptor gamma (PPARgamma) ligands and interleukin (IL)-6 are key factors for controlling prostate cancer cell proliferation and survival.Herein we used the natural PPARgamma ligand, 15deoxy Delta12-14 PGJ(2) (15dPGJ(2)), and IL-6 to define their interactions on proliferation and signal transduction in PC-3 cells. Cytotoxic and trypan blue exclusion assays, Western blot analysis of mitogen-activated protein kinases (MAPK) and Janus kinase/Signal transducer and activator of transcription (JAK/Stat) and real-time polymerase chain reaction (PCR) were methods employed as investigation tools.15dPGJ(2) reduced PC-3 cell proliferation, while IL-6 increased it. IL-6 induced PPARgamma expression but did not affect the PPARgamma ligand-mediated effects on the proliferation of PC-3 cells. However, 15dPGJ(2) inhibited the IL-6-mediated increase of PC-3 cell proliferation. 15dPGJ(2) activated Erk1/2 phosphorylation without affecting Akt phosphorylation and reduced phosphorylated and unphosphorylated Stat3 in PC-3 cells. IL-6 suppressed endogenous activation of Stat3 without affecting Erk1/2 and Akt phosphorylation and suppressed the 15dPGJ(2)-mediated activation of Erk1/2 phosphorylation in PC-3 cells.The interplay between PPARgamma ligands and IL-6 signalling could be important in controlling the growth of androgen independent prostate cancer cells as exemplified by PC-3 cells. |
| Databáze: | OpenAIRE |
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