Extensive junctional diversity in Ig light chain genes from early B cell progenitors of mu MT mice
| Autor: | L A, Bentolila, S, Olson, A, Marshall, F, Rougeon, C J, Paige, N, Doyen, G E, Wu |
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| Rok vydání: | 1999 |
| Předmět: |
Male
Mice Knockout B-Lymphocytes Reading Frames Immunoglobulin mu-Chains Stem Cells Immunoglobulin Variable Region Cell Differentiation Mice Transgenic Immunoglobulin Isotypes Mice Inbred C57BL Immunoglobulin kappa-Chains Mice Immunoglobulin lambda-Chains DNA Nucleotidylexotransferase Immunoglobulin J-Chains Lymphopenia Animals Gene Rearrangement B-Lymphocyte Light Chain Female |
| Zdroj: | Journal of immunology (Baltimore, Md. : 1950). 162(4) |
| ISSN: | 0022-1767 |
| Popis: | Nontemplated (N) nucleotide additions contribute significantly to the junctional diversity of all Ag receptor chains in adult mice except Ig light (L) chains, primarily because terminal deoxynucleotidyl transferase (TdT) expression is turned off at the time of their rearrangement in pre-B cells. However, because some Ig L chain gene rearrangements are detectable earlier during B cell ontogeny when TdT expression is thought to be maximal, we have examined the junctional processing of kappa- and lambda-chain genes of CD45(B220)+CD43+ pro-B cells from mu MT mice. We found that both kappa and lambda coding junctions formed in these B cell precursors were extensively diversified with N-region additions. Together, these findings demonstrate that Ig L chain genes are equally accessible to TdT in pro-B cells as Ig heavy chain genes. Surprisingly, however, the two L chain isotypes differed in the pattern of N addition, which was more prevalent at the lambda-chain locus. We observed the same diversity pattern in pre-B cells from TdT-transgenic mice. These results suggest that some aspects of TdT processing could be influenced by factors intrinsic to the sequence of Ig genes and/or the process of V(D)J recombination itself. |
| Databáze: | OpenAIRE |
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