Characterization of the tyrosyl radicals in ovine prostaglandin H synthase-1 by isotope replacement and site-directed mutagenesis
| Autor: | A, Tsai, L C, Hsi, R J, Kulmacz, G, Palmer, W L, Smith |
|---|---|
| Rok vydání: | 1994 |
| Předmět: |
Sheep
Base Sequence Free Radicals Indomethacin Molecular Sequence Data Electron Spin Resonance Spectroscopy Gene Expression Hydrogen Peroxide Transfection Recombinant Proteins Cell Line Substrate Specificity Kinetics Oligodeoxyribonucleotides Prostaglandin-Endoperoxide Synthases Mutagenesis Site-Directed Animals Tyrosine Amino Acid Sequence |
| Zdroj: | The Journal of biological chemistry. 269(7) |
| ISSN: | 0021-9258 |
| Popis: | Several free radical species, attributed to tyrosyl radicals, have been previously observed by electron paramagnetic resonance (EPR) spectroscopy during reaction of pure ovine prostaglandin H synthase-1 (PGHS-1) with hydroperoxide or fatty acid substrates. The identity and location of amino acid residue(s) involved in formation of these radicals has been investigated using wild type and mutant PGHS-1 expressed in transfected COS-1 cells. Upon reaction with hydroperoxide, detergent extracts from microsomes of cells expressing wild type ovine PGHS-1 rapidly produced EPR signals made up of a broad component, resembling either the wide doublet or the wide singlet seen in reactions of the purified ovine enzyme, mixed with a variable proportion of a narrow component. Isotope replacement with perdeuterated tyrosine and phenylalanine in wild type PGHS-1 caused no change in enzymatic activity or capability to form a peroxide-induced radical, but the radical was an isotropic singlet without any wide features. This loss of splitting in the deuterated enzyme establishes that the wide radical signal in PGHS-1 is indeed a tyrosyl radical, validating the earlier assignment based on spectral similarities with a tyrosyl radical in ribonucleotide reductase. A Y385F mutation abolished cyclooxygenase but not peroxidase activity, and the peroxide-induced EPR was narrowed to a singlet essentially identical with that obtained with indomethacin-treated ovine enzyme. Isotopic replacement with perdeuterated tyrosine and phenylalanine in the Y385F mutant led to further narrowing of the peroxide-induced EPR to an isotropic singlet, establishing that this radical also involved a tyrosine residue. A Y355F mutant exhibited some decrease in cyclooxygenase activity, but the peroxidase activity and the peroxide-induced radical characteristics were not significantly different from those of the wild type enzyme, indicating that Tyr355 is not involved in tyrosyl radical formation in PGHS-1.(ABSTRACT TRUNCATED AT 400 WORDS) |
| Databáze: | OpenAIRE |
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