Expression of beta-catenin and its mechanism of delocalization in intestinal-type early gastric cancer based on mucin expression
| Autor: | Soo Han, Lee, Hyun Jeong, Kang, Dong-Hun, Shin, Duk-Yeon, Cho, Jin Mi, Song, Han Cheol, Lee, Gwang Ha, Kim, Geun Am, Song, Mee Young, Sol, Jee Yeon, Kim, Kyung Un, Choi, Chang Hun, Lee, Gi Young, Huh, Do Youn, Park |
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| Rok vydání: | 2009 |
| Předmět: |
Adult
Male Genes APC Time Factors DNA Mutational Analysis Molecular Sequence Data Loss of Heterozygosity Polymerase Chain Reaction Gastrectomy Stomach Neoplasms Humans Promoter Regions Genetic Alleles Polymorphism Single-Stranded Conformational beta Catenin Aged Retrospective Studies Cell Nucleus Base Sequence Mucins DNA Neoplasm Sequence Analysis DNA DNA Methylation Middle Aged Immunohistochemistry Gene Expression Regulation Neoplastic Gastric Mucosa Mutation Female Polymorphism Restriction Fragment Length |
| Zdroj: | Histology and histopathology. 24(7) |
| ISSN: | 1699-5848 |
| Popis: | The biological characteristics of intestinal-type early gastric cancers (ICs) differ based on mucin phenotypes. Beta-catenin delocalization is a predictive marker of aggressive biological behavior (submucosal invasion and lymph node metastasis) of ICs. The presumptive causative genetic alterations leading to delocalization of beta-catenin in ICs are still controversial, and there are only a few reports regarding beta-catenin expression in gastric cancer based on mucin phenotypes. Therefore, in the current study, the expression and mechanisms of delocalization of beta-catenin were elucidated on the basis of mucin phenotypes in 109 cases of ICs. There was increased cytoplasmic and nuclear beta-catenin expression (delocalization) in ICs with a predominant intestinal mucin phenotype (ICIP; 46.3% [25/54 cases]) compared to ICs with a predominant gastric mucin phenotype (ICGP; 20% [11/55 cases]). There were no beta-catenin or APC mutations in ICs. APC promoter hypermethylation was present in 49 of 105 (46.7%) cases of ICs. There was a significant relationship between APC promoter hypermethylation and beta-catenin delocalization in ICs, especially in ICIPs. There was no relationship between beta-catenin delocalization and APC gene loss of heterozygosity in ICs. In conclusion, we showed that beta-catenin delocalization was more evident in ICIPs, and APC promoter hypermethylation might play a role in delocalization of beta-catenin, especially in ICIPs. |
| Databáze: | OpenAIRE |
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