| Autor: |
Zhang, Da-Long, Zhang, Shun-Wen, Cheng, Qing Hong, Wu, Fang, Wu, Jiang-Dong, Zhang, Jie, Dong, Jiang-Tao, Zhu, Hui-Yun, Zhang, Shuai, Wu, Qing-Qing, Zhang, Wan-Jiang |
| Jazyk: |
angličtina |
| Rok vydání: |
2017 |
| Předmět: |
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| Popis: |
Objective: To investigate the effect of peritoneal macrophage autophagy on immune function in sepsis mice. Methods: Seventy-two male BALB/C mice were intraperitoneally injected with LPS to induce sepsis. The mice were randomly divided into six groups: LPS+2 h, LPS+6 h, LPS+12 h, LPS+24 h and LPS+36 h. LPS with a dose of 10 mg/kg was intraperitoneally injected into the abdominal cavity of the sepsis mice, and the control group was injected with the same dose of saline. ELISA was used to detect the concentrations of inflammatory factors IL-2, IL-10 and TNF-α in the peripheral blood, and the CD4+T/CD8+T ratio in the peripheral blood was detected by flow cytometry. The expression levels of LC3II and Beclin-1/beta-action in the mouse macrophages were measured using Western blot to determine the level of autophagy. Results: The expression levels of LC3II and Beclin-1 were significantly higher in the peritoneal macrophages of the mice from the LPS+2 h group than in those of the mice from the normal group (P |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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