| Autor: |
Y S, Cheng, C E, Patterson, R G, Rucker, M J, Otto, C J, Rizzo, B D, Korant |
| Rok vydání: |
1992 |
| Předmět: |
|
| Zdroj: |
Advances in experimental medicine and biology. 312 |
| ISSN: |
0065-2598 |
| Popis: |
A 450 nucleotide sequence corresponding to the nucleotides 1931-2380 of the viral genome (8) was amplified by polymerase chain reaction (PCR) using template DNA prepared from HIV-2 (ROD) infected H9 cells. The sequence codes for HIV-2 protease and its N-terminal flanking peptide. An identical DNA sequence was obtained from three independent PCR amplifications, which differs from the published sequence of HIV-2 (ROD) in 7 nucleotides scattered throughout the region of the cloned DNA. The cloned DNA was expressed in E. coli cells and resulted in the synthesis of a correctly processed HIV-2 protease, which is enzymatically active. Therefore, none of the seven nucleotide changes, which resulted in two amino acid substitutions, affect the autoproteolytic or trans-cleaving activities of the HIV-2 protease. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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